14.13 Red cell folate (categories 2)

Red cell folate data available for STOP cohort only.

Over detection limit (360 to 1789 vs >=1790).

14.13.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	319.650574	1	<0.0001	***
rcfolate_gp2	4.746904	1	0.03	*
GA_samp	87.644077	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	33.315880	1	<0.0001	***
rcfolate_gp2	0.006379	1	0.9	ns
GA_samp	1643.003376	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	566.462807	1	<0.0001	***
rcfolate_gp2	1.024646	1	0.3	ns
GA_samp	761.783353	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp2	1	Inf	9.377	9.377	0.002	**
GA_samp	1	Inf	2.058	2.058	0.2	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp2	1	Inf	9.466	9.466	0.002	**
GA_samp	1	Inf	1.518	1.518	0.2	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp2	1	Inf	3.433	3.433	0.06	ns
GA_samp	1	Inf	1.092	1.092	0.3	ns

14.13.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	18.54 (13.47, 25.53)	<0.0001
rcfolate_gp2≥1790	1.11 (1.01, 1.21)	0.03
GA_samp	1.13 (1.10, 1.16)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.69 (1.42, 2.03)	<0.0001
rcfolate_gp2≥1790	1.00 (0.95, 1.06)	0.94
GA_samp	1.36 (1.34, 1.38)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.06 (0.05, 0.07)	<0.0001
rcfolate_gp2≥1790	0.96 (0.90, 1.04)	0.31
GA_samp	1.33 (1.30, 1.35)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	8.36 (5.71, 12.23)	<0.0001
rcfolate_gp2≥1790	1.22 (1.07, 1.39)	0.002
GA_samp	1.02 (0.99, 1.06)	0.15

HOMA-IR

Var	Effect	P
(Intercept)	1.80 (1.21, 2.70)	0.004
rcfolate_gp2≥1790	1.23 (1.08, 1.41)	0.002
GA_samp	1.02 (0.99, 1.06)	0.22

Glucose (mmol/L)

Var	Effect	P
(Intercept)	4.89 (4.57, 5.23)	<0.0001
rcfolate_gp2≥1790	1.03 (1.00, 1.06)	0.06
GA_samp	1.00 (0.99, 1.00)	0.30

14.13.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.13.4 Estimated marginal means

Outcome	rcfolate_gp2	response	SE	df	lower.CL	upper.CL
PRL (ng/ml)	360 to 1789	73.814	5.389	62.700	63.793	85.408
PRL (ng/ml)	≥1790	81.617	6.521	89.457	69.638	95.657
hPL (μg/ml)	360 to 1789	58.345	1.744	55.327	54.953	61.946
hPL (μg/ml)	≥1790	58.472	2.082	109.207	54.489	62.747
GH2 (ng/ml)	360 to 1789	1.458	0.046	74.897	1.369	1.553
GH2 (ng/ml)	≥1790	1.405	0.057	195.500	1.296	1.523
Insulin (uU/ml)	360 to 1789	11.045	0.332	Inf	10.414	11.715
Insulin (uU/ml)	≥1790	13.494	0.780	Inf	12.049	15.113
HOMA-IR	360 to 1789	2.324	0.074	Inf	2.183	2.474
HOMA-IR	≥1790	2.869	0.173	Inf	2.550	3.228
Glucose (mmol/L)	360 to 1789	4.721	0.029	Inf	4.664	4.778
Glucose (mmol/L)	≥1790	4.861	0.070	Inf	4.725	5.001

14.13.5 Plots

14.13.6 Post-hoc comparisons

Outcome	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	≥1790 / 360 to 1789	1.11 (1.01, 1.21)	0.03
hPL (μg/ml)	≥1790 / 360 to 1789	1.00 (0.95, 1.06)	0.9
GH2 (ng/ml)	≥1790 / 360 to 1789	0.96 (0.90, 1.04)	0.3
Insulin (uU/ml)	≥1790 / 360 to 1789	1.22 (1.07, 1.39)	0.002
HOMA-IR	≥1790 / 360 to 1789	1.23 (1.08, 1.41)	0.002
Glucose (mmol/L)	≥1790 / 360 to 1789	1.03 (1.00, 1.06)	0.06