14.13 Red cell folate (categories 2)

Red cell folate data available for STOP cohort only.


Over detection limit (360 to 1789 vs >=1790).

14.13.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 319.650574 1 <0.0001 ***
rcfolate_gp2 4.746904 1 0.03 *
GA_samp 87.644077 1 <0.0001 ***
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 33.315880 1 <0.0001 ***
rcfolate_gp2 0.006379 1 0.9 ns
GA_samp 1643.003376 1 <0.0001 ***
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 566.462807 1 <0.0001 ***
rcfolate_gp2 1.024646 1 0.3 ns
GA_samp 761.783353 1 <0.0001 ***
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp2 1 Inf 9.377 9.377 0.002 **
GA_samp 1 Inf 2.058 2.058 0.2 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp2 1 Inf 9.466 9.466 0.002 **
GA_samp 1 Inf 1.518 1.518 0.2 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp2 1 Inf 3.433 3.433 0.06 ns
GA_samp 1 Inf 1.092 1.092 0.3 ns

14.13.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 18.54 (13.47, 25.53) <0.0001
rcfolate_gp2≥1790 1.11 (1.01, 1.21) 0.03
GA_samp 1.13 (1.10, 1.16) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.69 (1.42, 2.03) <0.0001
rcfolate_gp2≥1790 1.00 (0.95, 1.06) 0.94
GA_samp 1.36 (1.34, 1.38) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.07) <0.0001
rcfolate_gp2≥1790 0.96 (0.90, 1.04) 0.31
GA_samp 1.33 (1.30, 1.35) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 8.36 (5.71, 12.23) <0.0001
rcfolate_gp2≥1790 1.22 (1.07, 1.39) 0.002
GA_samp 1.02 (0.99, 1.06) 0.15
HOMA-IR
Var Effect P
(Intercept) 1.80 (1.21, 2.70) 0.004
rcfolate_gp2≥1790 1.23 (1.08, 1.41) 0.002
GA_samp 1.02 (0.99, 1.06) 0.22
Glucose (mmol/L)
Var Effect P
(Intercept) 4.89 (4.57, 5.23) <0.0001
rcfolate_gp2≥1790 1.03 (1.00, 1.06) 0.06
GA_samp 1.00 (0.99, 1.00) 0.30

14.13.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.13.4 Estimated marginal means

Outcome rcfolate_gp2 response SE df lower.CL upper.CL
PRL (ng/ml) 360 to 1789 73.814 5.389 62.700 63.793 85.408
PRL (ng/ml) ≥1790 81.617 6.521 89.457 69.638 95.657
hPL (μg/ml) 360 to 1789 58.345 1.744 55.327 54.953 61.946
hPL (μg/ml) ≥1790 58.472 2.082 109.207 54.489 62.747
GH2 (ng/ml) 360 to 1789 1.458 0.046 74.897 1.369 1.553
GH2 (ng/ml) ≥1790 1.405 0.057 195.500 1.296 1.523
Insulin (uU/ml) 360 to 1789 11.045 0.332 Inf 10.414 11.715
Insulin (uU/ml) ≥1790 13.494 0.780 Inf 12.049 15.113
HOMA-IR 360 to 1789 2.324 0.074 Inf 2.183 2.474
HOMA-IR ≥1790 2.869 0.173 Inf 2.550 3.228
Glucose (mmol/L) 360 to 1789 4.721 0.029 Inf 4.664 4.778
Glucose (mmol/L) ≥1790 4.861 0.070 Inf 4.725 5.001

14.13.5 Plots

14.13.6 Post-hoc comparisons

Outcome Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥1790 / 360 to 1789 1.11 (1.01, 1.21) 0.03
hPL (μg/ml) ≥1790 / 360 to 1789 1.00 (0.95, 1.06) 0.9
GH2 (ng/ml) ≥1790 / 360 to 1789 0.96 (0.90, 1.04) 0.3
Insulin (uU/ml) ≥1790 / 360 to 1789 1.22 (1.07, 1.39) 0.002
HOMA-IR ≥1790 / 360 to 1789 1.23 (1.08, 1.41) 0.002
Glucose (mmol/L) ≥1790 / 360 to 1789 1.03 (1.00, 1.06) 0.06